Regulatory

Part:BBa_K2259015:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-01)


Toehold 2 of SynORI selection system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Toeholds required to adjust linker length in their sequence because the scar between basic parts shifts the protein reading frame.


Source

Acquired from supplementary material of Green AA, Silver PA, Collins JJ, Yin P. Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell. 2014;159(4):925-939.

References